This long-term soil and crop study in Iowa, with soybeans grown the second year, revealed that the yield was significantly improved by 2.32 bu/acre (4%) with Vitazyme. Soil and plant analyses results showed improvements in tissue contents of N, Mg, Ca, Fe, Mn, B, and, Cu. These values will be monitored each year as the study progresses, as will other physical, chemical, and microbiological parameters.