This Iowa long-term study revealed that Vitazyme, in the third year, increased the yield of corn by 3.9 bu/acre, while improving the quality of the corn considerably, especially the crude protein (0.45%- point), crude fat (0.36%-point), and ash (0.04%-point). Moreover, soil microbial populations were benefitted considerably with the product. These results continue to illustrate the positive effects of this product on both the yield and quality of corn grain, and on soil quality, in the U.S. Corn Belt.