In this Ukraine barley test, conducted on a gray forest-steppe soil, Vitazyme increased grain yield by 11% for one application and 24% for two applications. Likewise, grain quality was substantially improved by Vitazyme application, considering both “hatypa” and grain weight, especially with two applications. Income was likewise improved substantially, by 234 hrn/ha with one application and by 495 hrn/ha with two. These results show that Vitazyme in a highly viable crop input for barley farmers in Ukraine.